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Centrifuge rotor types and uses

Feb 22, 2020

The rotor is one of the important accessories of the centrifuge. Due to the high speed during work, the corresponding centrifugal force field is large, so the centrifugal rotors are made of high-strength aluminum alloy, titanium alloy, super-hard aluminum, and forged aluminum.

1. Angle turn
The holes for placing the centrifuge tube and the axis of the rotor have an inclination angle of 14 ° ~ 40 °, and generally 20 ° ~ 30 °. The smaller the inclination of the tube hole, the smaller the centrifugal force difference between the top and bottom of the centrifuge tube. This type of rotor is suitable for differential centrifugation. Can also be used for density gradient centrifugation, but the effect is less.

2. Shake the head
This type of rotor has a movable centrifugal bucket, which is placed in the bucket and suspended on the pin of the rotor. During centrifugation, the capacity of the centrifuge tube is much smaller than the angle of the rotor because the pin must withstand the large tensile force on it. When stationary, the bucket naturally sags and is parallel to the axis of the rotor. When rotating, the bucket swings outward with increasing speed and swings straight until it forms a 90 ° angle with the axis of the rotor. When this rotor is rotating, whether it is accelerating or decelerating, the centrifuge tube is always under the combined force of measuring force and centrifugal force, and the sample in the tube is not disturbed, so it is suitable for density gradient centrifugation. When used for differential centrifugation, the distance traveled by the sample particles is the length of the centrifuge tube, and the centrifugal force received increases linearly. When in use, first hang the balanced bucket according to its marked position, and then swing it by hand to see if it can rotate freely. After checking, it can run.

3. Zone rotor
The zone rotor has no centrifuge tube. The center of the rotor is a cavity, which is divided into several sections by several fan-shaped partitions. This type of rotor has a large capacity and can be sampled at low speed. It is designed for large-capacity density gradient centrifugation.

4. Turn your head upright
This type of rotor is similar to the angle rotor, and its main feature is that the hole where the rotor places the centrifuge tube is vertically downward and parallel to the axis of the rotor. When the rotor is rotated, the centrifugal force on the centrifuge tube is equal, and it is only suitable for differential centrifugation.

5. Continuous flow rotor
This type of centrifuge rotor is generally used for the concentration and separation of a large amount of culture liquid or raw liquid on a low-speed or high-speed centrifuge. The rotor has an inlet and an outlet. During centrifugation, the sample liquid continuously flows into and out of the rotor. Under the action of centrifugal force, the sample settles and separates in the centrifuge chamber. The separated particles sink into the edge of the rotor, and the supernatant flows out from the outlet. The advantages of this type of rotor are the continuous operation of the sample, the small volume of the rotor, the high rotation speed, and the good separation effect. At present, the common products include SorvaII continuous centrifugal rotors developed by the company, Beckman's JF-2 rotors, and GF-32Ti rotors. The speed can reach 6000r / min, which can be used for living cell separation.