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How to properly use and maintain the centrifuge

Mar 19, 2020

Centrifuges are a common tool in laboratories and hospitals, primarily used to separate and purify samples. High-speed rotation is used to make each particle in the centrifuge tube get an acceleration, and according to the density of the material in the sample, the separation and purification are performed. Because the particle needs to be supplied with a satisfactory acceleration, the speed of the centrifuge is very fast. Even the average low-speed centrifuge, the speed will be greater than the speed of many cars to travel normally. It is conceivable that such a fast speed, if we do not properly operate and maintain the centrifuge, it will be a very risky job. So today we will talk about how to properly use and maintain the centrifuge.

1. The centrifuge should be placed on a stable and solid surface when it is used. Large centrifuges should be placed on a solid floor and placed horizontally.

2. It is necessary to adhere to a certain safety interval around the centrifuge and not to store any dangerous items near the centrifuge.

3. Centrifuge is best not to exceed 60 minutes at a time

4. If abnormal phenomena are found during centrifugation, such as abnormal noise and oscillation, immediately press STOP (instead of turning off the power) to stop the inspection. Do not continue working until the cause is found.

5. During the operation of the centrifuge, do not open the lid of the centrifuge chamber, do not touch the rotating back and the centrifuge tube with your hands or foreign objects, do not bump or move the centrifuge, or rely on or sit on the centrifuge.

6. At the end of centrifugation, wait until the centrifuge is completely stopped before you can open the door, and quickly remove the centrifuge tube. First check whether the centrifuge tube is completely deposited and the deposition orientation.

7. After the machine is used, it should be cleaned well and keep the machine clean.

8. Select a suitable rotor, control the speed of the rotor, and the speed setting must not exceed the maximum speed. Reduce the maximum speed of the rotor in the following cases:
A: When the working time and number of times of the rotor reach the regular life;
B: When the rotor is exposed to local surface hazards or exhibits fine corrosion in the tube hole;
C: When using stainless steel centrifuge tubes, sleeves, thick-walled tubes or centrifuge bottles;
D: When the sample's uniform density exceeds 1.2g / cm3;

9. Before each use of the rotor, check the foreign matter and dirt in the hole severely, and if there is any harm, in order to maintain balance. After each use, the rotor should be soaked and cleaned with warm water (50 ℃ -60 ℃) and neutral detergent, and finally washed with distilled water or deionized water. After wiping dry with a soft cloth, use a hair dryer to dry, wax, and store dry.

10. The tensile strength and elongation of various centrifuge tubes are different and should be selected according to requirements.
A: Centrifuge tubes can be made from many different materials, such as polyethylene, cellulose, polycarbonate, polypropylene, etc. Take care to ensure that samples, solvents and gradient materials do not corrode the centrifuge tube and cap components during use;
B: Expired, aged, cracked or corroded centrifuge tubes should not be used or reduced as much as possible;
C: Control the use frequency of plastic centrifuge tube, pay attention to the standard equipment.